Peptide Reconstitution: Best Practices for Research Use

Peptides are supplied as lyophilised (freeze-dried) powders and require careful reconstitution before use. For optimal results, allow both the peptide vial and the chosen solvent—such as bacteriostatic water—to reach room temperature prior to mixing. This helps prevent cloudiness or precipitation that can occur when cold liquids are combined.

Managing Vial Pressure During Reconstitution

Due to the lyophilisation process, peptide vials may contain either a vacuum or slight positive pressure. To equalise this, insert a syringe partially filled with air into the vial’s rubber stopper. The syringe will naturally adjust the internal pressure by either drawing in or releasing air. This step helps prevent solution loss or spray-back when the solvent is introduced.

Additional Reconstitution Tips

  • Incomplete Dissolution

    If particles remain, sonication may assist in achieving full solubility.

  • Sterile Filtration

    To reduce microbial risk, consider filtering the solution through a 0.2 µm membrane.

  • Cold Storage

    After reconstitution, store peptides at 2–8°C. Under proper conditions, stability can be maintained for up to eight weeks.

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Conclusion

  • Proper reconstitution and storage techniques are essential for preserving peptide quality and ensuring reliable research outcomes. By following these guidelines, researchers can handle peptides with confidence and precision.