BPC‑157 is supplied as a lyophilised (freeze‑dried) peptide, sealed in a sterile glass vial to protect its stability and integrity. Before it can be used for research, it must be accurately reconstituted with a suitable mixing solution. This guide walks you through the full process, ensuring clarity, precision, and good laboratory practice.
What Is a Lyophilised Peptide?
Lyophilisation removes moisture from the peptide under vacuum, leaving a solid “puck” of stable material. This form helps preserve the peptide’s structure until it is reconstituted. Proper mixing ensures the peptide dissolves evenly and maintains its intended characteristics for research applications.
What You Will Need
- BPC‑157 5mg vial
- Mixing solution (e.g., bacteriostatic water)
- 1ml insulin syringes (29–30g recommended)
- Alcohol swabs
- Gloves
Note: 5mg = 5000mcg.
Preparation Steps
- Wash your hands thoroughly: Good hygiene helps prevent contamination.
- Wear gloves: Adds an extra layer of protection when handling vials and syringes.
- Allow materials to reach room temperature: Cold vials or cold mixing solution can affect the reconstitution process.
Opening the Vials
Flip off the plastic caps from both the BPC‑157 vial and the mixing solution. Clean the rubber stoppers with an alcohol swab to maintain sterility.
Equalising Pressure
Because lyophilised peptides are sealed under vacuum, pressure differences can occur. To avoid the solution spraying back:
- Draw half a syringe of air.
- Insert the needle into the BPC‑157 vial.
- Allow the vial to naturally equalise pressure (you may feel air push or pull).
How to Add the Mixing Solution
For this example, we will reconstitute the 5mg vial using 2ml of mixing solution.
- Draw 1ml (100 units) of mixing solution into the syringe.
- Insert the needle into the BPC‑157 vial at a slight angle so the liquid runs gently down the inside wall.
- Repeat the process to add a second 1ml, totalling 2ml.
Let the vial sit undisturbed until the peptide fully dissolves. Swirl gently if needed—do not shake vigorously.
Dose Calculations for Research
With 2ml added to a 5mg vial:
- 10 units (0.1ml) = 250mcg
- 20 units (0.2ml) = 500mcg
This allows for straightforward measurement using a standard 1ml insulin syringe.
Storage After Mixing
Once reconstituted, store the vial in a refrigerator to maintain stability. Avoid repeated temperature changes and keep the vial sealed when not in use.
Final Notes
This guide is designed to support researchers in preparing BPC‑157 accurately and safely. Proper reconstitution helps maintain peptide integrity and ensures consistent results in controlled research environments.
Frequently Asked Questions
Why is BPC‑157 supplied as a lyophilised powder?
Freeze‑drying protects the peptide from degradation and extends its shelf life until it is reconstituted.
Can I shake the vial to speed up mixing?
No—shaking can damage peptide chains. Always swirl gently if needed.
How should the reconstituted peptide be stored?
Keep it refrigerated and avoid unnecessary exposure to heat or light.
All products sold by Simply Peptides are strictly for laboratory research only. They are not intended for human consumption or medical use.